注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   癌症   >   WNT4 Antibody (Center)   

三百英雄吕布视频:WNT4 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
标注"皇冠"的产品,是满足高质量标准的产品。
了解更多...
  • 4 - WNT4 Antibody (Center) AP6683C
    Overlay histogram showing MCF-7 cells stained with AP6683c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6683c, 1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 4 - WNT4 Antibody (Center) AP6683C
    Overlay histogram showing MCF-7 cells stained with AP6683c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6683c, 1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - WNT4 Antibody (Center) AP6683C
    All lanes : Anti-WNT4 Antibody (Center) at 1:2000 dilution Lane 1: MDA-MB-231 whole cell lysate Lane 2: mouse bladder lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - WNT4 Antibody (Center) AP6683C
    All lanes : Anti-WNT4 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: human testis lysate Lane 3: mouse bladder lysate Lane 4: rat lung lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - WNT4 Antibody (Center) AP6683C
    Formalin-fixed and paraffin-embedded human bladder carcinoma reacted with WNT4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 产品详情
  • 文献引用 : 3
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, FC, E
Primary Accession P56705
Other Accession Q9QXQ5, P22724
Reactivity Human, Mouse, Rat
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 54361
Other Names Protein Wnt-4, WNT4
Target/Specificity This WNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-239 amino acids from the Central region of human WNT4.
Dilution FC~~1:25
WB~~1:2000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWNT4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WNT4
Function Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Plays an important role in the embryonic development of the urogenital tract and the lung (PubMed:15317892, PubMed:16959810, PubMed:18179883, PubMed:18182450). Required for normal mesenchyme to epithelium transition during embryonic kidney development. Required for the formation of early epithelial renal vesicles during kidney development (By similarity). Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries (PubMed:15317892, PubMed:16959810, PubMed:18182450). Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary (PubMed:15317892, PubMed:16959810, PubMed:18182450). Required for normal lung development and for normal patterning of trachael cartilage rings (By similarity).
Cellular Location Secreted, extracellular space, extracellular matrix
Research Areas
Effects of secreted frizzled-related protein 1 on proliferation, migration, invasion, and apoptosis of colorectal cancer cells.
Author : Wang Z#1,Li R#1,He Y1,Huang S1.
Cancer Cell Int. 2018 Mar 27;18:48. doi: 10.1186/s12935-018-0543-x. eCollection 2018.
29610564
Overexpression of miR?214 promotes the progression of human osteosarcoma by regulating the Wnt/β?catenin signaling pathway.
Author : Zhu XB1,Zhang ZC1,Han GS1,Han JZ1,Qiu DP1.
Mol Med Rep. 2017 Apr;15(4):1884-1892. doi: 10.3892/mmr.2017.6203. Epub 2017 Feb 14.
28260089
Estimated diversity of messenger RNAs in each murine spermatozoa and their potential function during early zygotic development.
Author : Fang P1, Zeng P, Wang Z, Liu M, Xu W, Dai J, Zhao X, Zhang D, Liang D, Chen X, Shi S, Zhang M, Wang L, Qiao Z, Shi H.
Biol Reprod. 2014 May 8;90(5):94. doi: 10.1095/biolreprod.114.117788. Print 2014 May.
24671878

BACKGROUND

The WNT family consists of secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT4 is a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. It play a concerted role in both the control of female development and the prevention of testes formation.

REFERENCES

Kuulasmaa,T., Horm. Metab. Res. 40 (10), 668-673 (2008)
Miyakoshi,T., Endocr. Pathol. 19 (4), 261-273 (2008)

FeedBack

终于等到您。ABCEPTA(百远生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-88856768 [email protected].


我要评价